Now available – Chapter 1: Sample Purification

Introduction to single-particle cryo-EM

Cryo-electron microscopy (cryo-EM) is a technique used to obtain 3D structures of biological macromolecules. The mission of CryoEM 101 is to provide self-paced learning material that introduces newcomers to the protocols, expectations, limitations, and troubleshooting of standard procedures of a typical cryo-EM project. To meet these goals, we offer media-rich content that uses illustrations, animations, narrated videos, and interactive training modules to cover major topics. The course is divided into five chapters: Sample Purification, Cryo-EM Grid Preparation, Cryo-EM Grid Screening, Data Collection, and Image Processing & Analysis.